Effect of recombinant Hp-FlgE2 of Helicobacter pylori on Nitric Oxide production by peripheral macrophage from BALB/c mice

Helicobacter pylori is a specific pathogen of the human stomach that immunomodulatory effects of Helicobacter pylori fractions have been suggested as an immune stimulus factor in vaccine candidate design. Helicobacter pylori FlgE2 protein is part of bacterial flagellum membrane whose effects on innate immune cells have not been studied. In the present study, we aimed to assess the effect of FlgE2 on the production of nitric oxide (NO) by rat peritoneal macrophages.Helicobacter pylori FlgE2 protein was recombinant produced. Peritoneal macrophages of mice were removed and cultured. Different concentrations of recombinant FlgE2 protein were used to stimulate macrophages and assess NO production. To detect NO, macrophage culture supernatant was removed and evaluated by reagent grease. Finally, the results were evaluated by SPSS software. The results showed that the recombinant FlgE2 protein from Helicobacter pylori increased the level of nitric oxide by increasing the concentration. At 80 μg/ml (P=0.01), the increase in nitric oxide level had the highest level of production and then was observed at 40 μg/ml, which increased significantly compared to the LPS control group. This increase was then observed at concentrations of 20 and 4 μg/ml.According to the findings of this study, recombinant FlgE2 has a positive effect on stimulation of NO production by peritoneal macrophages. Therefore, it is suggested that recombinant FlgE2 can be proposed as an immunostimulant for vaccine candidates.