Mutual effect of culture density and diet administration of pomegranate peel (Punica granatum L.) extracts on biochemical and antioxidant parameters of common carp (Cyprinus carpio) blood serum

Today, due to the confirmation of the existence of polyphenolic compounds with antioxidant properties in pomegranate peel, the study of its application potential in food health and fishery products has been the focus of researchers. In the present study, the effects of oral administration of ethanolic extract of pomegranate peel (Punica granatum L.) (Dose: 600 mg/kg) on functional changes of antioxidant system and biochemical parameters of blood serum of common carp (Cyprinus carpio) during a 45-day exposure against stocking density stress was monitored. In this regard, fish were randomly divided into 6 groups (3 treatment groups and 3 control groups with different levels of stocking density (3, 5, and 10 kg / m3) and three replications. At the end of the exposure period, fish bloodletting and serum preparation were performed. The results of this study did not show a significant increase in biochemical parameters (phosphorus, chlorine, magnesium, calcium, and triglycerides) as well as the activity level of some enzymes and antioxidant compounds such as SOD and MDA in fish serum compared to the control group. However, for stress indicators such as glucose and cholesterol and antioxidant activity such as CAT and GSH, production level has been significant. According to the findings, oral administration of pomegranate peel extract dietary supplement is recommended for consumption in dense aquaculture centers of common carp fish.