Viability of Human Adipose-DrivedStem Cells Seeded on Decellularized Amniotic Membrane Combined with Piperonylic Acid Induction

Adipose-derived stem cells (ADSCs) are the most common stem cell types used for treating a variety of diseases and also improve wound healing in preclinical and clinical trials. Human amniotic membrane (HAM) is one of the biological substitutes with specific potential to be applied in tissue engineering. Piperonylic acid (PA) is a small molecule extracted from black Piper nigrum that can activate signals associated with cell survival, growth, and proliferation.

To evaluate the viability and proliferation of cells, we decellularized HAM and human adipose-derived stem cells (hADSCs) seeded on DHAM inthe presence of piperonylic acid as a small molecule in culture media. Bax and Bcl2 gene expression as apoptosis-related genes was also studied by real-time PCR.

HAM decellularization was confirmed through the H&E and PI staining and DNA content assay. The viability and proliferation of hADSCs seeded onDHAM showed a significant increase in the presence of 75 μM PA. Cell cultivation on DHAM demonstrated significantly decreased Bax gene expression and increased Bcl2 gene expression.

Based on our findings, DHAM as a bio-scaffold and PA can improve the viability and proliferative potential of hADSCs in vitro. PA with growth factor-like properties can be used in various tissues engineeringand in vitro cell cultures.