The effect of 12 weeks of submaximal swimming training on MAPK and MDA biomarkers in rats exposed to tobacco-derived nitrosamine ketone

The purpose of this study was to investigate the effect of 12 weeks of maximal swimming training on MAPK and MDA biomarkers in rats exposed to tobacco-derived nitrosamine ketone.

48 6-8 week old Wistar rats with a weight range of 26 ± 105 g were purchased and randomly divided into 5 control groups (8 heads), distilled water injection(10 heads),NNK injection(10 heads), swimming training.(10 heads),swimming practice + NNK injection(10 heads),were divided.The training program included12 eeks of swimming with maximum intensity (five days a week). 48 hours after the last training session, lung tissue sampling was performed and the levels of MAPK and MDA markers of lung tissue were measured by ELISA technique. One-way ANOVA and Tukey were used to analyze the data

The results showed that the levels of MAPK(P = 0.001)and MDA(P = 0.000) were significant among the groups.The results of Tukey test showed that the reduction of MAPK in swimming and swimming +NNK intervention groups compared to NNK intervention group was significant(P = 0.001)(P = 0.001)and also, the rate of MDA reduction in swimming and swimming + NNK intervention groups was significant compared to NNK intervention group(0.001 = P)(001/0 = P).

The results of studies showed that swimming exercises can reduce the negative effects of NNK on MAPK and MDA levels to some extent, and it is possible that due to the anti-inflammatory role of swimming exercise activity can control the carcinogenic effect of NNK and protect the respiratory system in Equivalent to the carcinogen NNK.