Optimization Identification of wild mustard (Sinapis arvensis L.) seeds in rapeseed seed lots by morphological, chemical and Molecular methods

In the national standard for canola seed production, wild mustard is considered an illegal weed. Although canola and wild mustard can be distinguished by the morphological traits, it is not possible to distinguish the seeds of two species, especially the coated seeds. The present study was conducted to optimize the identification and detection of wild mustard seeds in rapeseed lots by morphological, chemical and molecular methods. The other seed count test was performed according to the rules of international seed test in rapeseed sampling. Chemical test design was performed under KOH treatment. The results of the chemical test were presented in five different status. Three specific markers of DA, DC and 5S rDNA were used, respectively. The Cruc marker is thought to be an internal control. Multiple polymerase chain reaction show amplification patterns of wild-mustard seed-specific markers with 5S rDNA amplified 190 bp that did not amplify in canola seeds. DA and DC markers in canola seeds amplified 239 and 625 bp, respectively, which did not amplify in wild mustard. According to molecular specific Profile and comparison with different status of rapeseed. Morphological limitation features and similarity of expression states of seed-related traits in wild mustard and rapeseed and factors such as seed coverage and chemical treatment restriction of wild mustard from rapeseed masses according International seed testing will not be possible, in which case DNA-based molecular tools will provide reliable results.