Investigation of the Antibacterial Effect of Selenium Nanoparticles and Sea Cucumber Extract on Staphylococcus aureus, Klebsiella pneumoniae, and Bacillus cereus bacteria

Many studies have been conducted in the field on the effects of industrial and chemical antibacterial substance abuse on human health and also the resistance of a wide range of bacterial species to antibiotics. Sea cucumber is one of the important aquatic animals which has therapeutic and nutritional properties. The purpose of this study is to investigate the antibacterial effect of selenium nanoparticles and sea cucumber extract on Staphylococcus aureus, Klebsiella pneumoniae, and Bacillus cereus bacteria.

The antibacterial activity of sea cucumber extract and selenium nanoparticles was investigated on three types of bacteria using the standard methods of disc diffusion, welling, determining the minimum inhibitory concentration, and determining the minimum lethal concentration. For comparing the antibacterial effects of these bacteria, the one-way ANOVA test and Scheffé test of multiple comparisons were deployed.

The results of this study showed that the methanolic extract of sea cucumber with a concentration of 10 mg/ml, as well as nano-selenium with a concentration of 1 mg/ml, and the combination of a concentration of 10 mg/ml of sea cucumber extract with 1 mg/ml of selenium nanoparticles has the best antibacterial effect on the three bacteria in question.

The combination of sea cucumber extract and selenium nanoparticles has a higher level of antibacterial properties.