Atresia ani is a developmental defect within the cloacal region, resulting in anal canal closure and abnormal routing of feces. There are four types of atresia ani including congenital anal stenosis (Type I), imperforate anus alone (Type II), imperforate anus with more cranial termination of the rectum as a blind pouch (Type III), Lack of contact between the cranial rectum, and terminal rectum (Type IV). Type II atresia ani is mostly combined with a rectovaginal fistula between the dorsal wall of the vagina and the ventral portion of the rectum. A 6-week-old female Persian cat was presented with anorexia, depression, and voiding of feces through the vulva, diagnosed with Atresia ani type II associated with rectovaginal fistula which was confirmed by radiographic examination with contrast medium. Surgical correction was performed under general anesthesia. The cat was able to control defecation and start to gain weight and no long-term complications were observed.