Single-Nucleotide Polymorphism of rs11061971 (+219 A>T) in Adiponectin Receptor 2 (AdipoR2) Gene and its Association with Risk of Type 2 Diabetes among Iranian Population

 Genetic modifications in the adiponectin receptor 2 (AdipoR2) gene can affect phenotypes associated with insulin resistance and diabetes. The purpose of this study was to evaluate the possible role of genetic modifications in the AdipoR2 gene, to determine the frequency of genotypes and polymorphism alleles of this gene at rs11061971 (+219 A>T), and to investigate its correlation with type 2 diabetes (T2D) and its related metabolic profile.

 In this case-control study, the single-nucleotide polymorphism (SNP) of AdipoR2 in 116 T2D patients and 102 controls was evaluated using RFLP PCR and FOK 1 enzyme. Fasting blood sugar, cholesterol, triglyceride, insulin, HDL-C, LDL-C and HbA1c were also measured and their correlation with the studied genetic modifications was assessed. The collected data were analyzed using Chi-square test and Hardy-Weinberg equation.

 There was a significant association in AT and TT genotypes in rs11061971 (+219 A>T) with T2D. However, no significant difference was observed in the frequency of alleles between the case and control groups. In addition, in LDL-C and total cholesterol in the control group, there was a significant difference between AA and TT genotypes as well as with AA and AT genotypes. However, no correlation was found between the other studied serum parameters and the genotype of individuals in the rs1106197171 polymorphism.

 It can be concluded that rs11061971 (+219 A>T) polymorphism is associated with T2D incidence. The findings suggest that AT and TT genotypes in this gene compared to AA genotype increase the risk of diabetes.