Evaluation of the Effect of Cold Argon Plasma and Platelet Gel on Biochemical Factors in the Wound Healing Process of Rats

Wound healing is a complex process in which a series of events takes place in a coordinated manner, which ultimately leads to tissue regeneration. One of the new treatment methods in medicine and veterinary medicine is the use of platelet-rich plasma (PRP) and plasma medicine.  The present study was conducted in order to compare the effect of cold argon plasma and platelet gel on the amounts of hexosamine, collagen and total protein in the tissue, in the wound healing process of rats; accordingly, 48 Wistar rats were used. After the adaptation period, the mice were classified into one control group and four treatment groups. The treatments included: nitrofurazone ointment treatment, platelet-rich plasma gel treatment, cold argon plasma treatment, and platelet gel treatment with cold argon plasma, and no treatment was performed in the control group. After the treatment and creation of skin wound, samples from the wounds were taken from the entire thickness between the shoulders of the rats, on days three, seven, 14 and 21 and collagen factors (hydroxyproline), hexosamine and total protein were measured. The results of measuring these three factors showed a significant difference between the control group and the plasma and plasma + PRP group (P<0/05). Meanwhile, no significant difference was observed between the control group and the nitrofurazone ointment group (P>0.05). As a result, it seems that the use of plasma and plasma + PRP accelerates the wound healing process significantly compared to the control group.