Evaluation of hypercin and hyperforin production in adventitious root line produced from Haypericum perforatum L. var. Topaz genotype under the influence of light spectra and different concentrations of IBA

For many years, different species of the genus Hypericum spp. used to treat neurological disorders and depression. The high susceptibility and fluctuation of the production of secondary metabolites caused by climate changes in this plant can be controlled by in vitro culture systems. Hairy roots (transgenic) has been used as an alternative for production of medicinal plants to produce secondary plant metabolites in many species, but the production of these roots have always faced challenges, one of them is the difficulty of induction. Therefore, the present study aimed to introduce practical guidelines for induction of adventitious roots from Hypericum perforatum leaf explants treated with different concentrations of indole-3-butyric acid (IBA) and different light sources including red, far red, full light, blue and flourcent , as well as absolute darkness as control treatment. The results of this study showed that the concentration of 2 mg/l IBA under dark treatment had the best inductive effect on adventitious root production from leaf explants. Higher concentrations of IBA caused callus formation in both light and dark conditions. In investigating the effect of light source on the frequency of adventitious root induction, the highest number of roots was recorded in far red and red, while the lowest number of adventitious roots was recorded under full light treatment. The highest level of hypercin was in the dark followed by fluorescent light and the lowest in blue light, but different light sources did not show a significant effect on hyperforin content.