The effect of Purslane seed supplement and aerobic training on mitochondrial biogenesis markers and DNA damage in kidney tissue in rats toxicated by hydrogen peroxide

Various treatment methods such as herbal supplements and lifestyle modification including regular exercise have been recommended to reduce oxidative damage and improve antioxidant defense. The aim of this study was to evaluate the effect of Purslane seed supplement and aerobic training on mitochondrial biogenesis markers and DNA damage in kidney tissue of rats toxicated by hydrogen peroxide.

To implementation of this experimental research, 54 male wistar rats randomly were divided into 9 groups. All groups received 100 mg/kg body weight of hydrogen peroxide (H2O2) for 14 days intraperitoneal. Rats in supplemented groups received hydrophilic Purslane seed with doses of 50, 200 and 400 mg per day by gavage method. Aerobic training was performed on a treadmill at a speed of 23 m/min, 30 min/day, 5 days/week for eight weeks. Data were analyzed using by two-way ANOVA and Bonferroni post hoc test at P<0.05.

The results showed that purslane seeds and the combined intervention of purslane seeds with aerobic training led to significant increase in the levels of O-6-methylguanine-DNA-methyltransferase and oxidant-prooxidant balance (PAB), as well as significant decrease the amounts of adenosine triphosphate (ATP) and malondialdehyde (MDA) in kidney tissue of rats toxicated by hydrogen peroxide (P=0.001).

It seems that aerobic exercise with purslane seeds can help to reduce the oxidative damage of kidney.