Antioxidant effect of copper nanoparticles coated with Artemisia annua plant extract in the 2nd degree burns in mice

Burns trigger intricate inflammatory processes and the generation of free radicals. The anti-inflammatory, antioxidant, and antibacterial characteristics of the Artemisia annua plant have a significant potential for enhancing the healing process for wounds. This study examines the impact of an Artemisia annua extract-coated copper nanoparticle ointment on the antioxidant enzyme activity in second-degree burns in mice. After the green synthesis of coated copper nanoparticles, 100 male mice were divided into five groups: control without treatment, eucerin ointment group, 0.2% copper nanoparticle ointment, 0.2% copper nanoparticles coated with Artemisia annua extract ointment, and 5% Artemisia annua extract ointment. The activity of antioxidant enzymes and the level of lipid peroxidation in tissue homogenates were measured on days 14, 7, 3, and 21. SOD, CAT, and GPX activities were all significantly higher on day 7 in the coated copper nanoparticle ointment group compared to the control group (P˂0.05). The level of MDA was also significantly lower in the control group on days 7, 14, and 21 (P 0.05). The extract of the medicinal plant Artemisia annua is useful for making and coating copper nanoparticles. In addition to the advantages of plant extract, copper nanoparticles have a synergistic impact that speeds up the healing of second-degree burn wounds in mice by lowering lipid peroxidation in wound tissue and boosting antioxidant activity.