The effect of eight weeks of propolis consumption during resistance training on the Glutathione peroxidase activity of kidney tissue of male rats consuming testosterone enanthate

Anabolic androgenic steroids are often used by athletes to improve sports performance. Kidney damage has been reported as a side effect of abuse of these substances. Therefore, the aim of the present study is to investigate the effect of propolis consumption during eight weeks of resistance training on Glutathione peroxidase activity in kidney tissue in male rats consuming testosterone enanthate.

In this experimental study, 32 male mice with an age of 8 weeks and an average weight of 250 ± 50 grams were prepared. after two weeks of familiarization and weighing twice a week, the mice were randomly divided into 4 groups: Control(C), sham(RT), resistance training +testosterone(RT+T) and resistance training+testosterone+propolis(RT+T+P) group were divided. The resistance training program was carried out 5 days a week with two rest days a week. The weights were 60% of body weight in the first week and 20% of body weight was added every week. Rats receiving steroids received 20 mg of testosterone enanthate intramuscularly. The receiving group received 400 mg of propolis cleanser by gavage. After dissection, kidney tissue was sampled to measure GPX concentration. Data analysis was done using one-way analysis of variance with SPSS24.

The results of one-way analysis of variance showed that there is a significant difference in the research groups (P=0.0001). According to Bonferroni's post hoc test, GPX levels in the exercise+testosterone and exercise+testosterone+propolis groups have significantly decreased compared to the control and sham groups.

It seems that testosterone consumption can be effective in increasing oxidative stress factors in the kidney tissue of resistance-trained rats. The use of propolis to treat the side effects of steroids has not had the desired effect in the short term.