New natural hosts and phylogenetic analysis of five Tomato yellow leaf curl virus isolates and associated satellites in Kerman province

Tomato yellow leaf curl virus (TYLCV) is one of the most important tomato infecting viruses worldwide. In this study, symptomatic plant samples of Moldavian dragonhead (Dracocephalum moldavica L.), broad bean, soybean, basil and euphorbia (Euphorbia sp. L.) were collected from Jiroft farms (Kerman province) in 2018-2019 and TYLCV infection as well as association of Tomato leaf curl betasatellite (ToLCB) and/or Gossypium darwinii symptomless alphasatellite (GDarSLA) with some of the samples were identified. Complete nucleotide sequences of the genome of five selected TYLCV isolates obtained from five plant hosts shared identities of 90-99% with each other and 88-97 with the selected GenBank isolates of TYLCV. Furthermore, the genome of ToLCB isolates from Moldavian dragonhead, broad bean, soybean and basil shared identities of 96-99% with each other and 94-95% with the counterpart betasatellite molecules from the GenBank database. Nucleotide sequence of GDarSLA, identified in soybean shared 93-98% identities with two Iranian GenBank isolates of GDarSLA. The above five plants are reported as new natural hosts of TYLCV, ToLCB and/or GDarSLA in Iran and also Moldavian dragonhead as well as broad bean are reported as new natural hosts of the virus in the world. Results of the current study indicate that TYLCV and the associated betasatellite have a wide distribution in Jiroft farms (Kerman province).