Evaluation of the frequency of rmtB and aac (6')Ib genes in strains resistant to gentamicin and amikacin in Klebsiella pneumoniae bacteria isolated from clinical samples ,Tabriz

The increase in antibiotic resistance of bacteria to aminoglycosides, especially Klebsiella pneumoniae, in recent years has caused concern among scientists and the World Health Organization. The aim of this study is to evaluate the rmtB and aac (6')Ib genes in clinical isolates resistant to gentamicin and amikacin antibiotics in Klebsiella pneumonia .

The current study is a descriptive-cross-sectional type, which includes 550 clinical samples from different wards of Alinasab Hospital in Tabriz. Identity of all isolates was reconfirmed using standard laboratory methods.The antibiotic resistance test for amikacin and gentamicin performed with using disc diffusion method based on CLSI standards. Also,The frequency of rmtB and aac (6') Ib genes was evaluated using the polymerase chain reaction.

In this study, 100 strains of Klebsiella pneumoniae were isolated from 550 clinical samples, 52% of which were women and 48% were men. The highest rate of resistance to gentamicin was observed with 34% and the highest sensitivity to amikacin was observed with 78%. Meanwhile, in the molecular results of polymerase chain reaction, 27.77% of isolates had rmtB gene and 16.66% had aac (6') Ib gene.

The results of the present study demonstrated that the increase of antibiotic resistance is a serious threat for human health. For this reason, in order to prevent the increasing prevalence of this risk, it is logical to optimize the use of antibiotics and culture in the society.