Investigation on the Effects of Carbon Nanotubes application on Regeneration of the Rosemary Plants under In vitro Conditions

Due to the distinctive physicochemical properties of the carbon nanotubes (CNTs) in enhancing photosynthesis efficiency, the aim of this study was to evaluate the effect of single-walled CNTs on morphological and physiological characteristics of rosemary. The experiment was performed in a completely randomized design with 10 concentrations of carbon nanotubes in 5 replications. The effect of CNTs on the morphological and physiological characteristics of rosemary was significant. Data means comparison showed that the highest height was observed at a concentration of 90 mg L-1 and the lowest was in control. The lowest regeneration percentage was in control. However, the results showed that the most and least leaf number was obtained from CNTs at the rate of 90 mg L-1 and control. The 90 mg L-1 concentration of CNTs had the highest leaf area and fresh weight. Also, the means comparison of physiological traits showed that the highest and lowest amount of chlorophyll a, chlorophyll b, carotenoid, flavonoid and total phenol were observed in CNTs at the rate of 90 mg L-1 and control, respectively. It is hoped that this method will be used for regeneration and the use of secondary metabolites in other plants.