Evaluation of the analgesic effect of platelet-rich plasma in the femoral head and neck excision in rabbits

The Femoral head and neck osteotomy (FHO) limits the contact between the femoral head and the acetabulum, and with the formation of dense fibrous tissue, allows the formation of a fibrous pseudo-joint in place. Platelet-rich plasma (PRP) contains platelets, growth factors and cytokines, which are involved in reducing joint pain and faster recovery of skeletal-muscular tissue. The purpose of this study is to investigate the analgesic effects of platelet-rich plasma in femoral head and neck ostectomy in rabbits. In this study, 18 New Zealand white rabbits were studied in two groups (n=9). Blood (6 ml) was taken from the heart of each rabbit separately and poured into tubes containing sodium citrate anticoagulant. To prepare PRP, primary centrifugation was performed at 1600 rpm for 10 minutes and secondary centrifugation at 2000 rpm for 10 minutes, and then it was activated by adding CaCl2. After complete anesthesia, femoral head removal was performed. In treated and control groups, the surgical site filled with PRP and normal saline, respectively. Finally, the joint capsule, muscles and skin were sutured in a routine manner. The studied groups were examined for symptoms of pain and lameness using the Glasgow system. Based on the results, in the PRP treated group compared to the control group, pain was significantly reduced, and as a result, it can be stated that the use of PRP in orthopedic surgeries can reduce pain. It leads to faster use of the organ by the patient and helps the patient return to normal conditions.