Simultaneous effects of Nanocide, Fungicide and Trichoderma fungi on the Canola Seedlings Affected by Stem Rot Pathogen, Sclerotinia sclerotiorum

In this research, the compatibility of Trichoderma species along with Nanocide and fungicide for the integrated management of this pathogenic fungi Sclerotinia sclerotiorum, has been studied. For this purpose, the effect of three isolates of Trichoderma harzianum, one isolate of T. virens, one isolate of T. atroviride and one isolate of T. koningii on the three isolates of pathogenic agent (SS1, SS2, SS3) in PDA medium containing 5 levels of Nanocide (0,30,60,100,130) by dual culture technique in the laboratory condition were studied. Then the effects of T. harzianum (1211), T koningii (iso2) and T. virens (6011) with two levels of Noniocid (10 & 30 ppm) and Iprodione+carbendazim fungicide (2 & 5 ppm) on one isolate of pathogenic agent in greenhouse condition, were evaluated. The lowest inhibition of mycelia growth of S. sclerotiorum in PDA medium with Nanocide, was 6%, that indicated for treatment S. sclerotiorum (SS2) × T. virens (6011) at level 10 ppm. In greenhouse condition, Trichoderma spp were tested in two stages, seed coated with Trichoderma conidia cell suspension and use Trichoderma mixed with soil. The evaluated parameters were height and wet and dry weight of the plant. The first experiment, was seed coated with Trichoderma conidia cell suspension, the most fresh weight was for T. harzianum (1211) as control and the most height was for T. koningii (iso2) alone. The second experiment, was for Trichoderma mixed with soil showed a significant increase in the most fresh and dry weight were for T. harzianum (1211) + 30 ppm Nanocide treatment and the most height of seedlings was also for T. harzianum (1211) + 2 ppm Iprodione–carbendazim (P≤0.01(. the statistical grouping showed that the treatments in which Trichoderma species were used alone showed no significant difference with treatments with Nanocid and fungicide in the wet and dry weight and height plant. Beside, using Trichoderma alone rather than the combination of Nanocid and fungicide, showed the better effect on growth parameters of the infected canola.