Investigating the Protective effects of vitamin D3 on the Physiological and histopathological changes of the testis in adult rats treated with Penconazole

Systemic triazole fungicides such as Penconazole have negative effects on different body tissues. In the Present study, the Protective effects of vitamin D3 on tissue changes and Pituitary-testicular axis in adult rats treated with Penconazole were investigated. In this experimental study, male Wistar rats were divided into 6 groups of 6 including: control, sham (1 ml of distilled water), exPerimental 1 (Penconazole 100 mg/kg), experimental 2 (vitamin IU/kg 1000 D3), experimental 3 (100 mg/kg Penconazole + 500 IU/kg vitamin D3) and experimental 4 (100 mg/kg Penconazole + 1000 IU/kg vitamin D3). At the end of day 28, serum levels of LH, FSH and testosterone hormones were measured by ELISA method and the testicular tissue was removed for histoPathological examination. The serum levels of LH and FSH in experimental group 1 showed a significant increase compared to the control and sham groups (P< 0.05), but the testosterone serum level decreased (P<0.05). The serum levels of LH and FSH in experimental grouPs 3 and 4 showed a significant decrease compared to experimental group 1 (P< 0.05), but testosterone serum levels increased (P<0.05). Histopathological examination showed that in experimental group 1, the testicular tissue was destroyed; however, in experimental groups 3 and 4, the testicular tissue was recovered. Based on the results of this study, vitamin D3 can improve testicular damage caused by Penconazole in rats.