Chickpea chlorotic dwarf virus: genome sequence of the virus from two new natural hosts, associated alphasatellite and demonstration of the pathogenesis in okra plant

Chickpea chlorotic dwarf virus (Mastrevirus, Geminiviridae) is a destructive geminivirus worldwide. In this study, symptomatic okra and wild jute samples showing typical geminivirus symptoms were collected from Jiroft farms and circular molecules of extracted DNA were enriched using rolling circle amplification (RCA) method followed by cloning and sequencing of RCA products of two selective samples from okra and wild jute. Sequence analysis showed that two isolates belong to strain CpCDV-F. Attempts to recover associated alpha- and betasatellite molecules from two plant samples using polymerase chain reaction assay resulted in detection of Gossypium darwinii symptomless alphasatellite (GDarSLA) only in wild jute sample. Sequence analyses indicated that two new CpCDV isolates shared nucleotide identity of 98% with each other and two viral and one alphasatellite sequences are closely related to Iranian Genbank isolates. Pathogenesis tests using previously constructed infectious clone of CpCDV led to appearance of yellowing and dwarfing in agroinoculated okra seedlings. Based on the results of this study, okra and wild jute plants are reported as new hosts of the CpCDV in Iran and wild jute is the new virus host in the world.