Investigating the Antibacterial Properties of the Hydroalcoholic Extract of Pomegranate Peel and Phenol on a Group of Intestinal Bacteria in Vitro

Pomegranate is a plant with an ancient history that has been widely used in medical sciences and its components have many properties. The aim of the present research is to synthesize pomegranate peel extract and investigate its antibacterial effects on a group of bacteria of intestinal bacteria and compare its antibacterial properties with phenol.

In this experimental study, pomegranate peel extract was extracted with the hydro alcoholic method. The Antibacterial effect of pomegranate peel extract was performed via disc diffusion and micro broth dilution methods on standard species of intestinal bacteria. All experiments were performed according to the guidelines of Clinical and Laboratory Standards Institute (CLSI). This study has been registered with the ethical code IR.KHALUMS.REC.1402.006 in the Ethics Committee of Khalkhal Faculty of Medical Sciences.

The MIC of pomegranate peel extract for Shigella dysentery, Escherichia coli, Klebsiella pneumoniae and Enterobacter aerogenes bacteria were 12.5%, 12.5%, 25% and 6.25%, respectively. Also, the MBC of pomegranate peel extract for Shigella dysentery, Escherichia coli, Klebsiella pneumonia and Enterobacter aerogenes bacteria were obtained 25%. The zone of inhibition for Shigella dysentery, Escherichia coli, Klebsiella pneumoniae and Enterobacter aerogenes bacteria was 18, 19, 16 and 22, respectively.

The results of this study has been showed that the hydroalcoholic extract of the pomegranate peel is very effective in the removal of intestinal bacteria in vitro. Also extract than phenol in lower contact time cause killing these bacteria.