Levels of Oxidative Stress and Inflammation in the Right Kidney After Left Ureteral Obstruction in Male Rats Treated With Troxerutin: An Experimental Study

Ureteral obstruction is one of the causes of kidney damage. Troxerutin is one of the strong natural antioxidants. The purpose of this study was to investigate the levels of oxidative stress and inflammation in the right kidney tissue after the left ureteral obstruction in male rats.

In this experimental study, 18 male Wistar rats were randomly divided into 3 groups (n=6 in either group) including control, ureteral obstruction, and ureteral obstruction+troxerutin. After the left ureter occlusion for 3-day, rats received troxerutin (100 mg/kg) daily 1h after occlusion for three days. Then, the right kidney weight and the blood urea nitrogen (BUN) level were measured. The malondialdehyde concentration, the glutathione peroxidase, and superoxide dismutase enzyme activity levels, as well as the tumor necrosis factor-alpha (TNF-α) expression level, were measured in the right kidney tissue. One-way ANOVA and Tukey's post hoc test were used to analyze the data.

Left ureteral obstruction caused a significant increase in the right kidney weight, malondialdehyde concentration, and TNF-α expression level (P=0.002) and a decrease in the activity levels of glutathione peroxidase (P=0.005) and superoxide dismutase (P=0.009) enzymes of ureteral obstruction group versus the control. Troxerutin was able to ameliorate these indicators in the ureteral obstruction+troxerutin group compared to the ureteral obstruction group (P<0.05). Blood urea nitrogen level mean did not show significant differenceacross the groups (P>0.05).

Left ureter obstruction induces inflammation and oxidative stress in the right kidney, and troxerutin consumption reduces inflammation and oxidative stress levels.