Exploring the Impact of Seasonal Sampling, Media Phase, Concentration and Type of Hormone on the Micropropagation of Dog Rose (<i>Rosa canina</i> L.)

One of the most significant decorative and medicinal plants, the dog rose (Rosa canina L.), serves as the rootstock for the majority of ornamental roses, including hybrid roses. The quick growth with desirable traits and the creation of wholesome, disease-free plants are the major outcomes of in vitro rose propagation. So, in order to provide micropropagation instructions for this plant utilizing nodal explants, this research was carried out. Many factors including the type, concentration, and phase of culture medium, sampling season, type, and concentration of hormones were investigated in several experiments. Explant disinfection was significantly affected by the timing of immersion in different sodium hypochlorite concentrations as well as the explant sampling season. The explants harvested in the winter and treated with 1% sodium hypochlorite showed the lowest rate of infection and the best percentage of survival. The percent of explant establishment was affected by the culture medium used as well as the IBA and BAP concentrations employed; the highest percentage of explant establishment was found in the MS culture medium with 0.3 mg L-1 BAP and or 0.4 mg L-1 IBA. The solid MS culture medium with 0.5 mg L-1 BAP had a stronger impact on proliferation. The results of the root formation test proved that the highest percentage of rooting is obtained in the half-strength MS culture medium with 2 mg L-1 IBA. After adapting to the environment with more than 80% survival, the produced plants were finally moved to the greenhouse. Using these findings, Rosa canina can be quickly commercially propagated for use in breeding and conservation efforts.