Determining the sequence and genetic diversity of the heat shock protein 70 gene in Iranian Adani goats using single strand spatial form polymorphism (SSCP) technique.
70 kDa heat shock proteins (HSP70) protect and maintain other proteins during heat stress. These proteins are a family of molecular chaperones that are needed for the correct folding of proteins and many cellular activities. Heat shock proteins (HSPs) are highly important due to their association with such economically important traits in animals as resistance to temperature shock and mastitis. This study aimed to amplify and investigate the polymorphism of the HSP70 gene in Adani goats. In the present study, blood was collected from 100 Adani goats from different regions of Bushehr province and DNA was extracted from the blood samples. Then, a fragment of 247 base pairs of HSP70 gene was amplified using PCR method and sequenced. Single strand spatial form polymorphism (SSCP) technique was used to determine the polymorphism. Agarose gel electrophoresis results confirmed the amplification of a 247 base pair fragment. Sequencing results showed three structural changes +130 (C130T), +163 (G163A) and +199 (G199C) in HSP70 protein of Adani goat compared to the goat reference gene with accession number JN604433. These mutations do not change the amino acid structure of HSP70 protein. The results of polyacrylamide gel electrophoresis showed that all the samples followed the same band pattern and there is no polymorphism. These results confirm that inbreeding is high in Adani goat herds and requires an effective breeding program to increase diversity in herds.