The Effect of Nickel on Seed Germination Proline Metabolism, Catalase And Peroxides Activity in Brassica Napus L

In this study, the effect of various NiCl2 treatments (0,10,50,100,150,200,250, 300,350, 400, 450,500 µM) on seed germination in Brassica napus cultivars (Hyola and PF) has been investigated. The results show that an increase in the nickel concentration causes a decrease in the seed germination in Hyola, which was greater than that in PF. Therefore, it is concluded that the toxicity of nickel is probably one of biochemical mechanisms influencing the response of these cultivars to the nickel stress. Also, the effect of various NiCl2 treatments (0,100,300,500µM) at rosette stage of two Brassica napus cultivars on the proline metabolism, catalase and peroxidase activity of leaves and root was investigated under the greenhouse conditions. The two types of cultivars (PF and Hyola) were highly sensitive to NiCl2 treatments. In general, in response to the heavy metal treatments, proline concentration, catalase and peroxidase activity in both varieties are increased. Therefore, it seems that the heavy metal stress could induce significant changes in proline and catalase and peroxidase activity both in the leaves and the root during the rosette stage through these cultivars.