A Method for Accelerating the Maturation of Toxocara cati Eggs

The effect of temperature and humidity on the maturation of Toxocara cati eggs in an in vitro system was investigated. Suspensions of Toxocara cati eggs, with 5% formalin/saline or 2.5% formalin/ringer were prepared and maintained at 37 °C under 40% humidity or at 25 °C under 98% humidity for 3 weeks for egg development. The suspension sample mixed by 2.5% formalin/ringer and maintained at 25 ºC and 98% humidity could fully embryonated the eggs of Toxocara cati in 3 weeks. The main advantage of this method is the increase of recovery and also reducing of the eggs maturation time.