Application of fermentor technology in production of diphtheria toxin

The present study shows the production of diphtheria toxoid in fermentor as comparedto old static method of production. With using 45l glass fermentor which has theprovision for growth of the organisms under controlled conditions with regard totemperature, pH, aeration and addition of maltose which can be automaticallymonitored and maintain microorganism growth toxin production and recording all ofthe conditions during growth. We succeeded to produce diphtheria toxin with 115 Lf(Rammon flucolation titer) within 40h time period in a minimum possible space, thenthe toxin was detoxified with formaldehyde, concentrated and purified and finallyquality controlled and compared with toxin produced with static method. Fermentorproduction showed a remarkable increase in volume, titer and immunogenecity in ashorter period of time, lower contamination and minimum labor work andrequirements. Therefore this technique can be industrially used for production ofdiphtheria Toxin (DT) and Diphtheria Pertussis Toxin (DPT) vaccines.