Effect of Dibenzo-18-crown-6 on haematopoitic cells colony formation of mouse bone marrow

Known biological effects of crown ethers influence us to investigate its effect in human body and use in drug production. The aim of this study was to evaluate the effects of Dibenzo-18-crown-6 on colony formation of bone marrow cells of mouse in culture medium and to find the probable mechanisms of the process. In this experimental study, red bone marrow cells of mouse (Balb/C) were used as source of haemapoietic stem cells. Culture of red bone marrow cells was done in the form of semi solid in the presence of different concentrations of crown ether, cAMP, PHO4H2K and PO4H2Na. Different concentrations of crown ether have significant inhibitory effects on colonizing of red bone marrow cells. The maximum inhibitory effect was observed in concentration of 80 mg/ml crown ether and no colony was found. The inhibitory effects of crown ether was diminished in the presence of cAMP with concentration of 10× 10-6 mol, PO4H2Na with concentration of 10× 10-6 mol and PO4H2K with concentration of 5×106 mol, but it couldn’t achieve the level of control group without presence of crown ether. HPLC method demonstrated that remaining level of crown ether reached to zero after sixth hour. The inhibitory effects of crown ether in control of colonizing the red bone marrow cells occur via decreasing of cations value and changing the physiological function of membrane resulting disorder in the cAMP signaling pathway.