An invitro evaluation of coronal seal of amalgam and C & B Metabond against bacterial microleakage

Improper coronal restoration after root canal therapy leads to penetration ofmicroorganisms along root canal or through voids of root canal filling to periapical tissues and ultimate failure. The aim of this study was to evaluate the sealing ability of amalgam and C&BMetabond against coronal microleakage of Entrococcus Faecalis. In this experimental in vitro study, 54 premolar teeth were randomly dividedto two groups of 24 teeth and for each the access cavities were prepared. The pulp chamber floorobturated with amalgam or C & B Metabond in two test groups. A further two groups of three teethserved as negative and positive controls. The external surface of each root, except the apical region,was covered with two layers of nail varnish. The teeth were inserted into Eppendorf plastic tubes andsuspended in glass vials containing sterile BHI broth. Entrococcus faecalis were placed in the accessof test and control groups and opacity of BHI was examined twice a week. The results were analyzedusing fissure exact test and ANOVA (E=0.05). Leakage was present in 13/20 of amalgam group; whilst 4/21 of the samples of C&B Metabondleaked at 3 months period. This difference was statistically significant and the sealing ability of twomaterials was increased by time lapse (p value < 0.05). C&B Metabond showed better sealing ability as a coronal seal thanAmalgam againstEntrococous Feacalis at 3 months period and its use is advised for double sealing before finalcoronal restoration.