Detection and genotyping of cutaneous leishmaniasis species in the southeast of Iran: restriction enzyme analysis (RFLP)

Leishmaniasis is a parasitic infectious disease which causes skin sores. There is no effective laboratory screening tests for leishmaniasis. Some diagnostic techniques exist that allow parasite detection and species identification by special culture and microscopy, biochemical (Isoenzymes), immunologic (immunoassays), and molecular (PCR) approaches. Specific major objectives of this study was to genotyping of Leishmania species in Bam and Shiraz city. A total of 83 samples of Leishmania were collected from patients clinically suspected of cutaneous leishmaniasis.