PADI-4 Gene polymorphism in patient with Rheumatoid Arthritis referring to Imam Reza Hospital in Mashhad

There are citrullin peptides in Rheumatoid Arthritis (RA) patients that are changed by Peptidyl Arginine Deiminase enzymes (encoded by PADI genes). An association between RA and PADI-4 haplotypes has been reported by researchers. The aim of this study is evaluation of PADI-4 Gene polymorphism in patient with Rheumatoid Arthritis referring. Methods and Materials: In this cross-sectional study we extracted the genomic DNAs from the whole blood samples of 50 patients with RA (on the basis of ACR criteria) referring to Emam Reza hospital in Mashhad and a control group involving 50 healthy khorrasanian participants. DNA genom was extracted with nonenzymatic salting out method. Genotypes were determined by PCR simple sequence specific primers (PCR-SSP). Data were analyzed with Chi-Square and Fisherexact Test. 58% of the patients had genotypes of 1and 2; 38%: 1,4, 2%: 1b,2, and 2%: 2,4. In the control group, 72% had genotype 1,2; 22%: 1 and 4, 6%: 1b,2 while none of them had genotype 2,4. There was not any significant relationship between the presence of different PADI-4 genotypes and RA in Khorrasanian population