Effect of Gamma-Ray Male Sterilization on the Integrated Control Management of Greater Wax Moth, Galleria mellonella L. (Lep., Pyralidae)

In this study, the control method of greater wax moth using male sterilization technique with gamma ray and chemical control methods were examined and compared. In order to determine the safe and effective dosage of gamma-ray to sterilize male pupae of the greater wax moth, an experiment was conducted in a complete randomized design with 5 treatments and 3 replicates with 50 male pupae in each replicate. Treatments included different levels of gamma-ray dosages with zero, 250, 300, 350 and 400 gray. Release ratio of sterile to normal males was also studied in a similar experiment. Treatments included sterile-males, normal males and virgin females with the ratios of 1:1:1, 2:1:1, 3:1:1, 4:1:1 and 5:1:1. In this study, male pupae were irradiated with 350 grays of gamma-ray. The possible parthenogenetic reproduction of this pest was also studied. In order to compare the results from the male-sterile technique and the chemical control of this pest, another experiment was conducted in wax stores using 3 treatments with 3 replicates. Phostoxin was used as the most common chemical pesticide with a dosage of 1 tablet of 3 grams of 56% per 1 m3 space for the control of this pest. The results showed that the best dosage to sterilize male pupae of the greater wax moth was 350 grays of gamma-ray. Also the best release ratio was four sterile male, one normal male for each normal female (4:1:1). Our results indicated that the females of the greater wax moth were not able to reproduce through parthenogenesis. There was no significant difference between the chemical and male-sterile methods for the control of this pest.