AN INVESTIGATION ON THE EFFECTS OF ANIONIC DETERGENT ON LIVER GLYCOGEN AND GLUCOSE IN COMMON CARP (CYPRINUS CARPIO)

Water pollution due to chemical substances as anionic detergents causes various disorders in aquatic ecosystems. We studied the effects of detergent on common carp using 6 treatments containing 0.5, 1,2,4, 6 and 8mg/l and one control. We used aquariums at a capacity of 150 liters and in each aquarium 8 pieces of carp 10 to 12cm long weighing 35 to 40mg each was introduced. Physical factors were measured with digital multi-meter unit CS535. Temperature and hardness of water were 23-24oC and 196mg/l, respectively. Air pump was used during the experiment for providing oxygen. Sampling was carried out to assay liver glucose and blood glucose every 24 hour during 7 days. Liver glucose concentration was determined by glucose oxidize enzyme and spectro-photometric method at 546nm length. Liver glycogen was determined after separation and hydrolysis with the same method as for glucose. Results showed that blood glucose was increased as a result of exposure to detergent, but liver glycogen was decreased when detergent concentration was higher than 2mg/l. A reverse correlation coefficient between the two factors affirmed the findings (P<0.001, r=-0.96).