The correlation between some biochemical and spermatological parameters in goldfish (Carassius auratus) semen

Some biological aspects of semen were investigated in goldfish (Carassius auratus), by determination of seminal plasma indices (ionic and organic composition) and their relationships with sperm motility. Seminal plasma contained 154.90±59.45 mmol/l Na+, 20.98±6.82 mmol/l K+, 0.69±0.42 mmol/l Ca+, 1.52±0.33 mmol/l Mg2+, 0.041±0.009 g/dl protein, 0.013±0.009 mg/dl cholesterol and 0.053±0.022 mg/dl glucose respectively. Semen spermatocriet was 52.83±11.44%, pH 8.81±0.35, motility duration 77.45±21.07s, percentage of motile spermatozoa 83.45±4.83%, sperm density 8.20×109 spermatozoa/ml and milt volume 082±0.43cc measured. There were positive significant correlation among spermatocriet, glucose and percentage of motile spermatozoa (r=0.765, r=0.832; P<0.01 respectively). But there was found significant negative correlation between spermatocrite and motility duration (r=-0.655; P<0.05). There were positive significant correlation among sperm density, pH and Mg2+ (r=0.648, r=0.660; P<0.05 respectively). Likewise there was found positive significant correlation between Ca+ and Mg2+ (r=0.807; P<0.01). The present study demonstrated that a significant correlation between seminal plasma ingredient and potential for motility in goldfish sperm.