Seroparasitological evaluation of Plasmodium vivax malaria and stability of the anti-plasmodial antibodies in Parsabad Ardabil province

Malaria is one of the most important parasitic diseases in the world and a major health problem in some areas of Iran. In addition to endemic areas in the south and south-eastern part of Iran a new threat of Plasmodium vivax malaria importation emerged from the Parsabad district which is located in Ardabil province in the north western part of the country. Malaria in this area may have originated from Azerbaijan Armenia or southern part of Iran. This study has been carried out to clarify seroparasitological results from Indirect Fluorescence Assay (IFA) stability of antiplasmodial antibodies and its comparison with those of confirmed direct microscopy in Parsabad district during 2003-2005. This seroparasitological study has been carried out on 250 samples from malaria infected patients which was previously confirmed by microscopy and treated with routine antimalarial agents and 250 samples of healthy control with no history of malaria in Parsabad during two years (2003-2005). Sera of collected blood samples were assessed for the presence of anti-plasmodial antibodies using IFA assay. Statistical analysis was applied by using ANOVA and Student’s t-tests with Graph Pad Prism. The results of this study indicated that all blood smears of test group were detected as positive by observation of P. vivax by direct microscopy and no positive smears were found among control group. Moreover no mixed-infection was observed among collected samples. In addition serological results revealed that 47 cases (19%) from test group and 4 cases (1.6%) from control group had antibodies against P. vivax malaria (P