Effects of Different Salinity Stresses on Osmotic Adjustment and Activity of Antioxidant-Enzymes in Two Sorghum Genotypes

To evaluate effects of different salinity levels on activity of antioxidant enzymes (CAT, APX and GPX) and osmotic components (carbohydrate and proline) in two sorghum genotypes, an experiment was conducted as completely randomized factorial design with three replications in Zabol university in 2007. Three levels of salinity control (0), 100 and 200 mM NaCl and two sorghum genotypes (Payam and Sistan) were compared. Results showed that by increasing salinity levels from 0 to 200 mM NaCl, the activities of these three antioxidant enzymes were significantly increased and the highest level of activity was observed in 200 mM salinity. Payam cultivar had the highest level of APX and GPX enzyme activity in these experiments. salinity significantly changed two osmotic adjustment concentrations (Carbohydrate and proline) and increased their concentrations. The highest proline and carbohydrate were showN in Sistan and Payam, respectively. In this study we found positive correlation between osmotic components and antioxidant enzyme activity. The results showed that in these two sorghum cultivars the two mechanisms help with salinity tolerance. A direct relationship between activity of the antioxidant- enzymes and accumulation of osmotic regulators (carbohydrate and proline) was observed. In Payam genotype, as the activity of the enzymes and accumulation of osmotic regulators increased, the weight of the plants decreased. The decrease in weight could mean some of plant energy is allocated to the production of antioxidant enzymes and osmotic regulators.