HBV genotypes in Golestan province of Iran

Background and ObjectivesTo date, almost eight HBV genotypes have been identified. HBV genotypes has special geographic distribution. They play an important role in trend and treatment of disease, progression into chronic phase, cirrhosis, and hepatocellular carcinoma. Clarification of viral genotypes may be useful in better understanding and predicting of the transmission pathways and disease prognosis. In the present study, we evaluated the genotypic disparity of HBV in Golestan province of Iran using PCR method. Materials and MethodsSerum samples from 126 HBsAg positive individuals were collected and stored in -20oC until the day of analysis. DNA extraction was carried out using standard phenol-chloroform method. For genotypic determination, we used HBV genotype specific primers. Electrophoresis of PCR products using ethidum-bromide stain, use of UV, and recording of the results were the next steps. ResultsOne hundred twenty six HBsAg positive serum samples were assessed. Suitable DNA samples were restricted to 117 out of 126; 93 (79.5%) out of 117 samples were detected as D genotype. ConclusionsThe most frequent type of HBV in our study was D type that usually causes a mild liver disease. So, proper vaccination, educational programs, and treatment with lamivudine are efficient strategies in controlling HBV infection in our area.