A Comparative Study of The Effects of Intraperitoneal and Intracerebellar Ventricle Injection of Picrotoxin and Biccuculine on Regulation of Blood Glucose Concentration in Mice

GABA is a inhibitory neurotransmitter in CNS that plays an important role in the regulation of blood glucose. There have been reports that GABA-A receptors might cause a reduction in the concentration of blood glucose by increasing the insulin plasma and decreasing the glucagon and somatostatine.

This study was carried out to compare the effects of interaperitoneal and intracerebellar ventricle injection of Picrotoxin and Biccuculine on regulation of blood glucose concentration in mice.

In this experimental study, male albino mice weighing 20-30 grams were used. Serial blood collection from each animal was done by retro- orbital sinus puncture, and glucose concentration was measured using O-toluidin. In this experiment, the effect of different doses (IP and ICV) injection of Picrotoxin and Biccuculine on blood glucose was studied. One-way variance analysis and t-test were adopted in statistical analysis of the collected data.

The results indicated that IP and ICV injection of Picrotoxin and Biccuculine induce significant increase of blood glucose concentration in two groups (p<0.05). Another point is that increased blood glucose concentration is observed until 45-th mintue in all doses but after this time, blood glucose almost according to similar pattern is reduced and in 90 minutes, it will reach its minimal level.

Therefore, it seems that GABA- A receptors have an inhibiting role in regulation of blood glucose. Further research seems inevitable since the effects of GABA-A receptors on blood glucose concentration is a complex phenomenon.