Investigation of disinfecting effect of AlproCid solution in dentistry

AlproCid is a brand new disinfectant which is a third Alkyl amine and ammonium with considerable effectiveness. The aim of this study was to evaluate of effectiveness of AlproCid on disinfecting dental surfaces and instruments. This laboratory-based study was conducted of 30 samples which were collected before and after administration of AlproCid from the same spots on dental instruments by using sterile swabs. The samples were cultured on blood agar and chocolate agar and were incubated in 37 oC for 24 hours.The standard bacteria were then identified to species level. The AlproCid solution was incorporated into strile paper discs. The effect of AlproCid was investigated by observation of growth inhibition zone around the disc and colony counting. The data was analyzed by using SPSS version 14 and Chi-Square test for determining the significance level and alpha error was set at 0.05%. From the total samples 86% were free of any bacteria. Microbial load was significantly reduced (P<0.05) after AlproCid application on dental instruments. The colony counting for all bacteria were reduced to less than 105, and the growth inhibition zone was at least 10 mm.This in vitro study showed the effectiveness of AlproCid on laboratory standard strains of E. coli, Klebsiella, Proteus, Entrococci resistant to vancomycin, and S. aureus resistant to methicillin. This effect was bactericidal. AlproCid is an effective agent in decreasing bacterial contamination of dental instruments without any adverse effect on the surface of instruments. The solution could be used for easy and rapid disinfecting of all surfaces.