Enrichment and Isolation of Lytic Bacteriophages Against Antibiotic-Resistant Pseudomonas aeroginosa

One of the most common problems in the hospitals is the appearance of the resistant infections to the antimicrobial agents. Bacteriophages can use as an alternative to antibiotics for preventing and treating bacterial infections. Bacteriophages are viruses that infect and kill bacteria. They are safe; having no activity against animal or plant cells and appears to have evolved with bacteria as they are ubiquitous in nature. The aim of present study was isolation and enrichment of bacteriophages againstantibiotic resistant Pseudomonas aeruginosa isolates. Clinical samples from patients hospitalized in different wards of Ghaem Hospital (Mashhad, Iran) were collected and 43 antibiotic-resistant Pseudomonas aeruginosa were obtained from the samples. Appropriate lytic bacteriophages were isolated from refined sewage of the septic ward of the samehospital and bacteriocidal effect of the bacteriophages was evaluated in both liquid and solid phases. All tests were three times repeated in different bacteriophage concentrations under different conditions.The results were analyzed using statistical mann-witney test. By using two methods; solid and liquid phase the high concentration of prepared bacteriphages showed complete bactericidal effects on antibiotic-resistant isolated bacteria (p<0.0001). Our data showed the high efficacy of bacteriophages disinfectant effects and it was more prominent in the higher concentration of the bacteriophages.