Effect of Bio-Gen and Bio-Oss with and without Alendronate on bone formation by Guided Bone Regeneration: A histomorphometric study in dogs

Several different graft materials, e.g. autologous, allografts, alloplasts or xenografts have been used to preserve or reconstruct the ridge anatomy. The purpose of this study was to investigate histologically and histomorphometrically experimental defects that grafted with Bio-Gen and Bio-oss and influence of local delivery of Alendronate with graft materials on bone formation. This experimental study did in 5 dog’s tibia, 7 round intrabony defects,6mm in diameter and approximately 4 mm in depth were made with trephine bur, each defect in each tibia filled randomly with following groups: 1) Bio-Gen (Bio-teck)+BCG membrane, 2) BBM(Bio-Oss)+Bio-gide membrane, 3)Bio-Gen+ alendronate+BCG membrane, 4) Bio-oss+alendronate+Bio-gide membrane, 5) BCG membrane, 6) Bio-gide membrane, 7) Control (empty). Animals developed euthanized after 3 months. Immediately after euthanasia the tibia bones were dissected out and the proximal part of the tibias containing the defects were removed in blocks and prepared for Histologic and Histomorphometric evaluation. The data were analyzed using the ANOVA and Tukey procedures. P-values were less than 0.05. Newly formed bone was well evident in all of the defects; inflammatory cells were less than 10% in all of them. The mean percentage of new bone in these 4 groups (with grafted materials) was higher than the other ones (with membrane and without grafted materials) and control groups (p<0.05). There is no statistical different between Bio-Oss and Bio-Gen groups (with or without alendronate) in vital bone percentages. Application of single dose alendronate combination with Bio-oss or Bio-Gen granules doesnt improves bone formation. Bio-Gen granules are considered an osteoconductive graft material suitable for regeneration of bone.