Regeneration of Miniature Rose (Rosa hybrida,. cv Tanbakeda') Through Somatic Embryogenesis

Rose is considered as one of the most popular and important ornamental plants in the world. During the last decade, tissue culture, micropropagation as well as genetic engineering have prevailed in rose production industry. Gene transfer to embryogenic callus, derived from different types of explants is currently one of the most popular methods of improvement of rose cultivars, presented in particular as cut flower with such novel characteristics as blue color. An efficient tissue culture procedure covering high frequency embryogenic callus induction and plant regeneration is pre-requisite for every successful genetic engineering program. An efficient method for embryogenic callus induction from growing nodal stem segments on MS medium followed by a selection of appropriate young leaflets is hereby reported. MS medium complemented with 2mg/L 2,4-D, and 0.5mg/L NAA as wekk as medium B5 complemented with 0.5mg/L 2,4-D and 2.5mg/L NAA plus 0.5mg/L kinetin were used for callus induction. Cali were then subcultured in MS medium with higher levels of hormone (3 mg/L 2,4-D and 0.5 mg/LNAA) to develop suitable size and shape embryos. This was followed by transferring the calli onto an MS medium complemented with 8 hormonal treatments. MS medium along with 1 mg/L of TDZ resulted in a highest number of embryos. High frequency of shoot development from mature somatic embryo and subsequent plant regeneration were obtained on a medium containing 3 mg/L of BAP. Shoots were rooted on MS medium supplemented with three various hormonal treatments, and there were no significant differences observed between between treatments.