The effects of ovafact (GnRHa) on biological characteristics of semen in commons, comets, wakins and fantails goldfish (Carassius auratus gibelio)

In this study، the effect of hormon Ovafact (GnRHa) on biological semen characteristics (motility duration، percentage of motile spermatozoa، spermatocrit، sperm density، milt volume، pH، Na+، K+، Ca+، Mg2+، total protein، cholesterol and glucose) in sub species commons، comets، wakins and fantail goldfish (Carassius auratus gibelio) males was compared. From each treatment ten males has been investigated. Fish injected with with 10μg kg−1 GnRHa combined with domperidone. There was a highly significant difference of seminal plasma pH among treatments (p<0/01)، as the highest value of pH observed in commons treatment (8. 88±0. 42). There were a highly significant difference in percentage of motile spermatozoa and motility duration among treatments (p<0/05) as the highest value percentage of motile spermatozoa and motility duration observed in commons treatment (83. 65± 21. 05، 88. 19±5. 65 respectively). While the highest value of spermatocrit (%) and sperm density (×109 number) observed in waking and fantail treatments (53. 38± 5. 66، 7. 81± 1. 58 respectively). Also the highest value of milt volume observed in commons treatment (0. 92± 0. 46 ml). But there were no significant difference in Na+، K+، Ca+ among treatments (p>0/05)، While the highest value of Mg2+ observed in fantail (1. 57±0. 26 mmol/l). Likewise there were no significant difference in total protein and cholesterol among treatments (p>0/05)، While the highest value of glucose observed in fantail (0. 3± 0. 33 mg/dl). The present study demonstrated that GnRHa have been more effective on biological characteristics of semen in commons and fantail than comet and wakins.