The Effect of Platelet-rich Plasma (PRP) and Bio-oss on Bone Regeneration in Furcation Class II Defects: A Histologic and Histomorphometric Study in Dogs

Statement of Problem: Periodontal regeneration in the furcation defect is considered as one of the most challenging aspects of periodontal therapy. Among different approaches, GTR has shown promising biologic and clinical results. There are is evidences indicating that the use of platelet-rich plasma (PRP) may improve the outcomes of regenerative periodontal therapy. The purpose of this This study was aimed to examine the effect of the combination of platelet-rich plasma (PRP) and Bio-oss on bone regeneration in class II furcation defects in dogs. 48 experimental defects with 5 mm horizontal and 5 mm vertical open probing depth were created in furcation areas of each premolars of dogs. The defects were randomly divided into 3 groups: test group in which the defects were grafted with bio-oss and PRP; control group in which the defects were grafted with bio-oss alone; and negative control group in which no graft was used. An reabsorbable collagen membrane was placed over each defect area site and the flaps were sutured. The animals were sacrificed 3 months later and biopsy samples were taken from the defect sites for histologic and histomorphometric assessment. In each section, the area fractions (%) within the bone specimens occupied by newly formed bone, soft tissue, and residual grafting particles were determined. The data were analyzed statistically using Student's t test through SPSS software (version 13.0). The Bone bone fill was 61 % in the test group, 58 % in the control group, and 39 % in the negative control group. With Although no significant difference was observed between the test and control groups, the differences between the test and control groups with negative control group were significant (p <0.05). In the control group, samples the lamellar bone formation was higher and the chronic inflammatory infiltration was lower. Within the limitations of this study, the use of bio-oss and PRP in the treatment of class II furcation involvement has no additional benefit over bio-oss alone. However, it seems that PRP can improve the quality of the new bone while decreasing the amount of tissue inflamation.