Molecular Epidemiology of Staphylococcus aureus Isolated from patients and personnel in Army hospital

staphylococcus aureus has emerged over the past several decades as a leading cause of hospital- and community acquired infection. A significant component in the success has been its acquisition of antibiotic Resistant factors.We have developed a rapid and simplified approach for the strain characterization of staph.aureus on the basis of multilocussequence typing. Method and material: MLRFT for s.aureus involes amplification of seven house keeping gene locus.the amplicons are then digested directly with one or two restriction Enzyme and the restriction fragments are resolved by agarose gel electrophoresis. These isolates had been additionally characterized for their susceptibilities to antibiotics. MLRFT resolved 146 isolates into 19 RFT that 48 isolates have the same molecular patterns. Their susceptibilities to antibiotics were showed 90% resistance to methicillin. MLRFT provides convenient procedures for molecular epidemiology it requires minimal laboratory facilities and is relatively simple and inexpensive to perform.63% of cluster (24 isolates) take placed two fold cluster that 90% of them was resistant to methicillin, 55% belong to twofold cluster, there fore methicillin resistant isolates can transmit easily.