Callogenesis and organogenesis of Chrysanthemum morifolium Ramat L

The effects of different cytokinins (benzyl amino purine(BAP), Kinetine (Kin) incombination with different auxins(Indole butyric acid(IBA), 2,-dichlorophenoxy aceticacid (2,4-D) were investigated on callogenesis and organogenesis of Chrysanthemummorifolium Ramat L. Leaves explants were cultured in the MS medium supplementedwith different auxins and cytokinins. Results of using IBA and BAP in the media indicatedthat calli which were taken from medium with IBA (2 mgL-1) and BAP (0.5mgL-1) produced roots and callus of IBA (1 mgL-1) and BAP (1mgL-1) produced shoots.By using 2,4-D and Kin in the medium, rhizogenesis were occurred in the media supplemented with 2,4-D (0.5, 4 mgL-1) and Kin (2 mgL-1).Subculture of calli increasedrooting. Calli of medium with 2,4-D (0.5 mgL-1) and Kin (2mgL-1) produced shoot afterthe first subculture in the free hormone medium. In the 2,4-D and BAP treatments,maximum level of callogenesis and rhizogenesis were achieved. Calli in the mediumsupplemented with 2,4-D (0.5 mgL-1) and BAP (1mgL-1) produced roots and shoots.