Histological study of the interactions between blastema tissue originated from the pinna of New Zealand white rabbit and natural 3D elastic scaffold in vitro

The aim was to investigate the interactions and cellular behavior of the blastema tissue and natural 3D elastic scaffold in vitro. In this study cows aorta was used as a scaffold. To prepare a very porous elastic scaffold, the cells and collagen were removed by treating that with 50 mg/ml cyanogen bromide in 70% formic acid. The prepared scaffold were then placed inside the blastmea rings and kept in culture media for 40 days. The interaction between blastema tissues and elastic scaffolds were studied in 10 days intervals. Microscopic studies in different day on blastema tissue and scaffold revealed that the cells and collagen fibers were omitted successfully from the elastic scaffold. Moreover, histological studies indicated that the cells had penetrated into the scaffold. In addition cell devision, probable differentiation of blastema cells to fibroblast and myocyte and also angiogenesis due to inductve effect of elastic scaffold were abserved. Our results indicated that, it is possible to prepare a natural elastic scaffold from aorta by treatment with cyanogen bromide. On the other hand, this scaffold may have inductive effects on cell behaviors such as migration, adhesion, cell division and probably differentiation. Further studies are required to confirm the indentity of cells and other properties of the scaffold and also its possible use in engineering of vascular tissue.