Investigation of Decolorization of Reactive Black 5 by Enzymatic Method

This research reports on the examination of enzymatic decolorization. Decolorization of Reactive Black 5 using fungal peroxidase obtained from Coprinus Cinereus was studied in a stirred tank reactor. Effect of several parameters including hydrogen peroxide concentration، pH، dye concentration and enzyme activity on the decolorization of Reactive Black 5 was investigated. Dye decolorization efficiency of 67% was achieved under continuous feeding of dye solution and stepwise addition of hydrogen peroxide and peroxidase enzyme. Under this condition، pH value، dye and hydrogen peroxide concentrations were 8. 0، 40 ppm and 96. 1 mM، respectively.