Serological and molecular detection of Citrus psorosis virus in Golestan province

In recent years, symptom of psorosis on stem oak leaf pattern on citrus trees leaf appeared in Gorgan areas. In order to identify of causal agent (Citrus psorosis virus), sampling was done from infected orchard of Gorgan area during 2006-2007. Sampling was done from all of citrus cultivars in this area including sweet orange (Yafa, Washengton novel, Sunkin and Tompson) and Mandarin (Clemantin) frome suspicious trees to infection that has shown psorosis diseases symptoms. Serological test was done via DAS-ELISA using of monoclonal antibody of CPsV and RT-PCR test was done via specific primer of CPV1 and CPV2 that designed from coat protein gene cod area of CPV on 200 samples was collected from Gorgan area orchards and six samples from Citrus Research Center (Ramsar) scientific glasshouse that show specific glasshouse that showed specifice Symptoms in Biological Indexing. DAS-ELISA testsdid not show any infection by using CPsV monoclonal Antibody (Agritests®, Italy). The primers (CPV1 and CPV2) were produced on a 600 bp cloned fragment of CPsV coat protein gene. Laboratory tests showed an infection of 7.5% samples commercial groves and the all samples from Citrus Research Center (Ramsar) scientific glasshouse. No relationship was observed between the commercial CPsV monoclonal antibody and CPsV infection. However, results showed that the best RNA extraction method is the guanidine buffer. This is the first report of CPsV occurrence in Golestan province.