Study of probable genes controlling sexuality in date palm (Phoenix dactylifera L.) by DNA marker with arbitrary primers

Date Palm (Phoenix dactulifera L.) is monocotyledon, diociouse and long life which is higheconomic important in Iran. Since fertility date palm starts after five years, sexualitydetection is important in first growth section, accelerate breading programs and when palm grove create. Therefore program for selecting a useful date palm need to know molecular markers. In this study used of 16 arbitrary primers with 9 to 21 bases for amplified template DNA. It is used of 30 date palm cultivars (20 male cultivars and 10 female cultivars. Two genome bulks made in two 1.5 μl microtubes. HB9, OPO08, A12 and K1 primers was produced band only in DNA mixed of male cultivars and there wasnt any band from these four primers in female bulk. HB12, HB7 and 814 primers was produced band only in DNA mixed of female cultivars. Then HB9, OPO08, A12 and K1 primers was tested in 20 male cultivars which three primers of HB9, OPO08 and K1 was produced 350, 1100 and 450bp respectively in all male cultivars. Three primers of HB12, HB7 and 814 were tested 10 female cultivars separately and HB7 primer was produced 700bp same band in all female cultivars. Stability of these bands demonstrated their ability in determination of date palm sexuality in several repeats and cultivars which had genetic distance.